Researchers at Chalmers University of Technology in Sweden have developed a method for labeling mRNA molecules that helps them track them in real-time using a microscope.
RNA therapy opens up a whole range of new possibilities for the prevention and treatment of disease.
However, the delivery of RNA-therapeutic drugs into the cell does not work now. For new treatments to reach their potential, it is necessary to optimize the delivery of drugs to the human body. The new method, coined by researchers at Chalmers University of Technology, could help science move from traditional drugs to RNA-based therapies.
The study, which forms the basis of the scientific article, scientists conducted together with chemists and biologists from Chalmers University, employees of the biopharmaceutical company AstraZeneca and a research group from the Pasteur Institute in Paris.
The method involves replacing one of the building blocks of RNA with a fluorescent version: it can be used to obtain information about the movement of RNA (mRNA) without affecting the body’s work process. In addition, fluorescence allows researchers to track functional mRNA molecules in real-time and observe how they enter cells. All this can be done with a microscope.
The big advantage of this method is that now we can easily see where exactly the mRNA enters the cell and where the protein is formed. We do all this without losing the natural ability of RNA to translate proteins.
Elin Esbjerner, Associate Professor of the Department of Biology and Biotechnology and the second lead author of the article.
Researchers in this field can use the method to learn more about the learning process. This will help open up more new drugs. The new method complements the knowledge of modern methods of studying RNA under a microscope.